It is vital to avoid contamination during pipetting in order to obtain reliable results. This article examines three types of pipetting contamination: pipette-to-sample contamination, sample-to-pipette contamination, and sample-to-sample contamination, as well as ways to help you improve your pipetting processes.
Pipette-to-Sample Contamination
Because of the purity of the pipette tip, contamination can occur when a contaminated Pipette or Pipette Tip contaminates the sample.
Pipette tips are classified into three types based on their purity degree:
Heavy metals, UV stabilizers, antioxidants, pigments, mold releasing agents, biocides, and surfactants may leach from non-sterile pipette tips. High quality tips made from 100 percent pure polypropylene do not contain leachate in high quality manufacturing facilities. While contaminants such as DNA enzymes, RNA enzymes, and endotoxins are difficult to eradicate using any sterilizing process, the existence of these contaminants usually necessitates independent testing by a third party laboratory. If you require high purity pipette tips, we advise that you confirm that they have been tested with your tip supplier.
Pipettes can contaminate samples in regular laboratory work if these basic principles are :
Sample-to-Pipette Contamination
Contamination occurs when liquid or aerosol particles from the pipette enter the pipette's body.
To reduce sample contamination by the pipette,the following precautions are recommended:
Sample-to-Sample Contamination
Sample-to-sample contamination occurs when aerosol or liquid residue from one sample is carried over to the next sample. To avoid contamination: