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What Can You Do to Prevent Pipetting Contamination?
September 08 , 2021

It is vital to avoid contamination during pipetting in order to obtain reliable results. This article examines three types of pipetting contamination: pipette-to-sample contamination, sample-to-pipette contamination, and sample-to-sample contamination, as well as ways to help you improve your pipetting processes.

 

Pipette-to-Sample Contamination

Because of the purity of the pipette tip, contamination can occur when a contaminated Pipette or Pipette Tip contaminates the sample.

Pipette tips are classified into three types based on their purity degree:

Pipette Tips Manufacturer


  • Non-sterilized
  • Sterilized and free of microorganisms
  • Certified free of contaminants such as DNA enzymes, RNA enzymes and endotoxins


Heavy metals, UV stabilizers, antioxidants, pigments, mold releasing agents, biocides, and surfactants may leach from non-sterile pipette tips. High quality tips made from 100 percent pure polypropylene do not contain leachate in high quality manufacturing facilities. While contaminants such as DNA enzymes, RNA enzymes, and endotoxins are difficult to eradicate using any sterilizing process, the existence of these contaminants usually necessitates independent testing by a third party laboratory. If you require high purity pipette tips, we advise that you confirm that they have been tested with your tip supplier.

Pipettes can contaminate samples in regular laboratory work if these basic principles are :


  • Use a positive displacement filter or a filter tip. Aerosols are prevented from entering the pipette body and potentially contaminating following samples by using the filter.
  • After each sample, always replace the pipette tip.
  • On a regular basis, autoclave or sanitize pipettes or parts that may come into touch with the sample.


 

Sample-to-Pipette Contamination

Contamination occurs when liquid or aerosol particles from the pipette enter the pipette's body.

To reduce sample contamination by the pipette,the following precautions are recommended:


  • Always slowly release the pipette button, to avoid aerosol generation and uncontrolled liquid splashing at the pipette tip.
  • During pipetting, keep the pipette vertical and store the pipette upright. This keeps liquid from entering the pipette body.


 

Sample-to-Sample Contamination

Sample-to-sample contamination occurs when aerosol or liquid residue from one sample is carried over to the next sample. To avoid contamination:


  • Use a filter tip or volumetric filter. Alternatively, a filter on the pipette tip cone can be employed.
  • After each sample, always replace the pipette tip.


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