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Tissue Cassette: Minimum Bearing Temperature and Embedding Precautions
February 08 , 2022


  • Question: I want to use liquid nitrogen for treatment, but I don't know if the plastic Embedding Cassettes can still be alive at - 169 ℃?
  • Answer: Freeze samples in liquid nitrogen first and embed them when slicing.


Our tissue cassette is made of POM material. The recommended temperature for POM material is - 40 ° C ~ + 100 ° C.

 

After the tissue is fixed, dehydrated, transparentizing, and soaked in wax, the process of wrapping it into the wax block or plastic block containing tissue block with embedding agent (such as paraffin, resin, plastic, etc.) is called embedding. Different embedding methods have different requirements. After embedding, the tissue can reach a certain hardness and toughness, which is conducive to cutting into ideal slices.

 

Embedding Precautions

1. Each time the tissue block is clamped, the tweezers shall be heated to a moderate temperature with an alcohol lamp.

2. There shall be no water, broken wax, and other foreign matters in the embedding bracket, so as not to affect the embedding quality.

3. The temperature of embedded wax shall be well controlled, and the melting point of embedded wax shall be 58 ~ 60 ℃.

4. Embedding wax and adding a small amount of beeswax (beeswax) can increase the toughness and facilitate slicing.

5. When clamping the tissue block into the embedding bracket, be sure to pay attention to the embedding surface.

6. When the embedded wax block is trimmed with a knife, the white wax edge around the tissue in the wax block must be properly reserved to facilitate continuous slicing.

7. After embedding a piece of tissue, the forceps must be wiped clean or burned with an alcohol lamp.

 

Superfirt Histology Tissue Cassettes

Embedding Cassette Wholesaler

Embedding Cassettes in Superfirt

These disposable Embedding Cassettes are very effective in preserving tissue samples during embedding. These boxes are made from a special high-density polymer(POM), which can safely immerse the specimen in the liquid and completely resist the chemical action of the solvent. Efficient flow channels maximize fluid exchange and ensure proper drainage. The integral cover does not require a separate steel cover.

Our 30 ° and 45 ° Embedding Cassettes have a large writing surface and two side panels, which are very suitable for labeling.

Embedding Cassettes Bulk at a competitive price. Free OEM/ODM Design, & Logo Imprint. Get A Quote.

Learn more about our tissue cassettes'types, parts, and maintenance. Read this blog>>>Tissue CassetteWhat You Need to Know 

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